Configuration has been changed
Sometimes, when I close a gel processing window, the program asks me "Configuration has been changed. Do you want to save the changes?" What does this apply to?
Any type of data that can be translated into a densitometric curve is considered a fingerprint type in the BIONUMERICS and GelCompar II software. This includes commonly used genotyping methods employing agarose or polyacrylamide slab gel electrophoresis (PFGE, rep-PCR, RAPD, PCR-DGGE, etc.), in which case the data are usually imported as two-dimensional gel images (bitmaps). Another major group consists of capillary electrophoresis profiles such as AFLP, ARISA, T-RFLP, etc. Here, the raw electropherograms generated by an automated sequencer (genetic analyzer) or derived peak table text files can be imported. Finally, any other profile (generated e.g. by gas chromatography, HPLC or spectrophotometry) that can be seen as peaks or bands, can be analyzed as a fingerprint.
Sometimes, when I close a gel processing window, the program asks me "Configuration has been changed. Do you want to save the changes?" What does this apply to?
In the normalization part of the software, the curves in the right panel are very flat, with peaks almost invisible. Can I change this?
How important is the delineation of a gel by the green rectangle?
What automated sequencers is BioNumerics or GelCompar II compatible with?
When I open a TIFF file the program tells me "Invalid TIFF file". What is wrong?
Is it possible to perform quantification studies in GelCompar II with the combination of the Basic Software and the Cluster Analysis module?
How do I convert my data from GelCompar 4.2 into BioNumerics or GelCompar II?