Nucleic acid or amino acid sequences, from import and assembly over multiple alignment to annotation, SNP analysis, primer design and comparison of complete chromosomes.
In this tutorial we will screen a set of whole genome sequences of some Staphylococcus aureus samples for the presence of the mecA sequence. The assembled sequences present in the whole genome demonstration database of Staphylococcus aureus are used in this tutorial.
In this tutorial we will screen genome sequences of Escherichia coli samples for phenotypic traits using the E. coli functional genotyping plugin. This plugin contains public databases for serotype, virulence and resistance prediction, as well as plasmid and prophage detection. An in silico PCR tool is also implemented, making it possible to detect Shiga toxin gene subtypes and virulence genes, mimicking the wet lab PCR.
This tutorial illustrate how to merge two (or more) sequences and store the joined sequence in an appointed sequence type experiment.
This tutorial illustrates how to compare whole genome sequences in a BIONUMERICS database.
In this tutorial similarity/distance based trees and phylogenetic trees (maximum likelihood and maximum parsimony trees) are calculated for a set of aligned sequences. Different groups are defined in the Comparison window giving a nice overview of the relationships, the clusters present in the database and the outliers.
In this tutorial you will learn how to annotate multiple sequences with a single action based on an annotated sequence available in the BIONUMERICS database.
This tutorial demonstrates the annotation of genomes by Prokka[1] available on the Calculation Engine.
[1] Torsten Seemann. Prokka: rapid prokaryotic genome annotation. Bioinformatics, 30(14):2068-2069, 2014.
This tutorial illustrates how to annotate a single sequence based on one or more annotated sequences and how to edit the annotation and perform a BLAST analysis.
This tutorial illustrates how to edit and analyze an individual sequence in the BIONUMERICS Sequence editor. Analysis tools include frame analysis, restriction enzyme analysis, primer design and BLAST analysis.
This tutorial illustrates how to fetch and import sequences from online databases such as EBI and NCBI into a BIONUMERICS database.