In this tutorial we will focus on the first two steps of the wgSNP workflow in BIONUMERICS: reference sequence selection and mapping of the reads against the reference sequence. The mapping of the reads against the reference sequence is done on the cloud calculation engine.
Demonstration database containing data for a set of 97 Staphylococcus aureus isolates originating from three published studies. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.