In this tutorial we will focus on the last two steps of the wgSNP workflow in BIONUMERICS: 1) wgSNP analysis + filter out relevant SNP, 2) wgSNP clustering.
PDF file:
Download demonstration database:
WGS_demo_database_for_Staphylococcus_aureus
Demonstration database containing data for a set of 97 Staphylococcus aureus isolates originating from three published studies. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.