In this tutorial we will screen whole genome sequences of Mycobacterium tuberculosis complex samples for phenotypic traits using the Mycobacterium tuberculosis complex functional genotyping plugin. Phenotypic traits include: spoligotype, lineage and antibiotic resistance. The plugin also allows you to perform species confirmation.
Demonstration database containing data for a set of 29 Mycobacterium tuberculosis complex isolates. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.